1. Basic situation of the project

1. Project name: Spatial proteomics detection technology services

2. Project number: EBS26081500003

3. Project location: Jinfeng Laboratory

4. Procurement method: inquiry

2. Procurement content

3. Business requirements

1. Delivery time: Complete all experiments and data analysis work and complete acceptance within 30 days after signing the contract.

2. Supply/service location: Jinfeng Laboratory, No. 313 Jinyue Road, Chongqing High-tech Zone.

3. Payment method: Settlement will be carried out after the final report is delivered and accepted, and the entire service fee will be paid in one go after formal settlement.

4. Total project price limit: The total quotation price shall not exceed the limit price of 80,000.00 yuan. The quoted price is a comprehensive lump sum price, including all equipment fees, software fees, testing fees, travel expenses, freight, material fees, sample return fees, taxes, etc. The purchaser will not pay other fees separately.

4. Project requirements

Party B shall complete spatial proteomics testing, data processing and results delivery within the 15 ROI areas confirmed by the purchaser based on the 5 brain glioma tissue samples provided by the purchaser. The service content should cover sample and pathological information verification, ROI confirmation, regional separation, low-input protein preparation, mass spectrometry detection, quality control, data analysis and technical support.

The technical route should be customized according to the sample type, tissue area and the purchaser's scientific research problem.； Without written confirmation from the purchaser, ROIs may not be merged or replaced, key technical routes may be changed, and only fixed templates or software-generated reports may be provided.

Project specific requirements:

(1) Sample and ROI management

1. There are 5 cases of brain glioma samples in this project. In principle, 3 ROI areas are selected for each case, totaling 15 ROIs.； The final area shall be subject to the marking confirmed by the purchaser based on H&E and adjacent section staining results.

2. Party B should check the sample number, slice information, ROI number and grouping information, and establish a one-to-one correspondence from the sample, ROI, mass spectrometry original file to the result file to ensure that the entire process is traceable.

3. Sample completeness, tissue area, removable volume, and low-input detection feasibility should be evaluated before formal processing.； If the sample is found to be damaged, insufficient in area or other problems that may affect the test, a written list of problems should be formed in a timely manner and the solution should be confirmed with the purchaser.

4. Before formal batch testing, Party B should confirm the sampling area, number of slices, ROI boundaries and testing process with the purchaser； If necessary, select a representative sample for process verification, and then implement it in batches after confirmation by the purchaser.

5. Each ROI should be independently sampled, prepared and tested independently.； Different ROIs or different samples may not be mixed without the written consent of the purchaser.

(2) Spatial sampling and low-input sample preparation

1. According to the pathological area confirmed by the purchaser, use laser microdissection (LCM) or an equivalent area separation method approved by the purchaser to complete ROI sampling, and try to avoid the mixing of non-target tissues.

2. Carry out protein extraction, quantification, enzymatic hydrolysis and necessary peptide processing for trace amounts of tissue. The process used should be suitable for tissue samples with low input space and minimize sample loss.

3. The sampling method, estimated area or tissue volume, number of sections, sample consumption and abnormal conditions of each ROI should be recorded and delivered together with the project results.

4. When the test may fail due to insufficient sample quality or tissue quantity, the purchaser should be informed before continuing to consume the sample.； It is not allowed to collect additional materials or dispose of remaining samples without confirmation.

(3) Mass spectrometry detection and quality control

1. Use a high-sensitivity liquid chromatography-tandem mass spectrometry detection process suitable for low-input tissue samples to complete protein identification and quantification of 15 ROIs. Suppliers should describe the main instrument platforms, acquisition modes and key quality control measures in the technical plan.

2. Appropriate process quality control or instrument quality control should be set up during the testing process to evaluate chromatographic stability, mass spectrometry response, identification quantity, quantitative completeness and repeatability, and record abnormal batches and processing conditions.

3. Samples from the same comparison system should try to use the same pre-processing process, detection platform and collection parameters.； When batch testing is indeed necessary, the batch arrangement should be explained and potential batch effects evaluated.

4. All original mass spectrometry data, search records and quality control results should be kept intact, and only filtered results should not be delivered.

(4) Data processing and analysis

1. Complete protein identification, quantification, data filtering, missing pattern assessment, necessary normalization and quality control to form a traceable ROI × protein quantification matrix.

2. Carry out data distribution, correlation, PCA, hierarchical clustering, outliers and necessary batch effect assessment at the sample and ROI levels.

3. Carry out differential protein, expression trend and regional specificity analysis according to the regional grouping confirmed by the purchaser, and provide effect size, P value and multiple test correction results.

4. Carry out GO, KEGG, Reactome, GSEA, PPI network or other appropriate function and pathway analysis based on data quality and research questions. Do not form conclusions based solely on a single enrichment P value.

5. The analysis should combine pathological region information to form a complete logic from regional differences and key proteins to functional modules and biological explanations.； If the sample size does not support a complex prediction model, this should be stated truthfully and no over-modeling or exaggerated conclusions should be allowed.

6. The software name and version, database version, key parameters, filtering rules and statistical methods should be provided to ensure that the core results are repeatable and reviewable.

(5) Delivery of results

Party B shall at least deliver:

1. All mass spectrometry raw data, protein identification results and quantitative matrix；

2. Correspondence table of samples, ROI, original files and result files, as well as sampling records and descriptions of abnormal situations；

3. Complete result table and source data for data quality assessment, statistical analysis, difference analysis, function and pathway analysis；

4. PDF, SVG vector images and TIFF and PNG images of no less than 300 dpi that can be directly used in scientific research reports and paper drawings；

5. Complete project analysis report, methodology description, software/database version and key parameters；

6. R/Python analysis script or equivalent analysis process that can reproduce the core results；

7. Without affecting testing and quality control, remaining samples, sections or extracts shall be returned or disposed of as required by the purchaser.；

8. The original data, analytical data, charts and derivative results generated by the project belong to the purchaser.； Without the written permission of the purchaser, it shall not be disclosed, transferred or used for other research, commercial databases and algorithms, or artificial intelligence model training to third parties.

(6) Technical services and acceptance

1. The sampling, testing, quality control and analysis results of 5 samples and 15 ROIs should be complete, with clear and traceable correspondence.； If the objective sample cannot be completed due to objective sample conditions, original records, cause analysis and handling opinions confirmed by the purchaser should be provided.

2. After the regular analysis of the project is completed, no less than 3 rounds of personalized data analysis, result review or chart optimization services should be provided according to the scientific research needs of the purchaser.

3. Unqualified results caused by errors in Party B’s sample processing, mass spectrometry testing, statistical methods, program codes or analysis processes shall be re-tested, analyzed or corrected free of charge.

4. The acceptance is based on the procurement announcement, technical solutions confirmed by both parties, samples, ROI corresponding records, quality control reports, delivery lists and the reviewability of core results. Regular automated reports shall not be used to replace the agreed results.

(7) Documents proving ability to perform the contract

1. The supplier should provide a technical implementation plan that matches this project in the quotation document, describing the proposed regional sampling method, low-input protein preparation process, mass spectrometry platform, quality control measures, data analysis process, and the project leader and core technical personnel to be invested.

2. In order to prove its ability to perform the contract, the supplier should provide one of the following materials:

(1) Proof of completed proteomics or spatial omics-related technical service projects from January 1, 2023 to the quotation deadline, including key pages of the contract and one of the completion, acceptance or customer performance certificates.

(2) Proteomics or spatial omics-related cooperation results completed by the supplier or the person in charge or core technical personnel who intend to invest in the project, including the homepage and DOI of the paper, search information, or other result materials that can prove actual participation in related work.

3. The certification materials should be complete, clear and stamped with the official seal of the supplier. The contract amount and sensitive information unrelated to this project can be concealed, but key information used to verify the ability to perform the contract, such as the parties to the contract, service content, signing time and signature, must not be concealed. The purchaser has the right to verify original documents or public information.

4. This item does not limit the administrative region, industry, contract amount or testing platform brand of the customer.； Material content should be directly relevant to the low-input proteomic detection and data analysis capabilities required for this project.

(8) Quotation materials and subject certification

1. The business license or other organization certification documents should be complete, clear, and within the validity period, and a color scanned copy should be provided. All information and signatures stated in the certificate in accordance with the law should be complete and legible. Cropping, blocking, missing pages, or only partial screenshots should not be provided.； The copy must be stamped with the official seal of the supplier.

2. The supplier’s name, unified social credit code, legal representative, signature subject and authorization relationship in the quotation document should be consistent. If the subject certification or authorization materials are incomplete, unclear, unable to be verified or the signature is missing, it will be treated as an invalid quotation.

(9) Review of abnormally low price response

According to the "Notice of the Ministry of Finance on Promoting the Solution to the Problem of Abnormally Low Prices in Government Procurement" (Caiku [2026] No. 2), if one of the following situations occurs during the review, the abnormally low price response review should be initiated:

1. The response quote is 50% lower than the average response quote from all suppliers that have passed the compliance review；

2. The response quote is 50% lower than the response quote from the second lowest quote supplier that passed the compliance review；

3. The response quotation is 45% lower than the maximum price of the project；

4. Based on professional judgment, the review team believes that the quotation is too low and may affect the quality of the inspection or other circumstances in which the contract cannot be performed in good faith.

After initiating the review, the relevant supplier shall submit a written explanation of the reasonableness of the quotation and necessary supporting materials within a reasonable time specified by the review team (in principle, no less than 30 minutes), including but not limited to cost estimates for sample processing, reagent consumables, instrument time, labor, data analysis and quality control, as well as corresponding personnel, equipment and contract performance arrangements.

If the supplier fails to submit explanations and supporting materials, or the materials submitted cannot prove the reasonableness of its quotation and feasibility of contract performance, the review team shall treat it as an invalid response.； The reasons, comments and results of the review should be recorded and archived.

5. Supplier qualification requirements

Meet the qualifications of Article 22 of the "Government Procurement Law of the People's Republic of China":

1. Have the ability to independently bear civil liability.

2. Have good business reputation and sound financial accounting system.

3. Have the necessary equipment and professional technical capabilities to perform the contract.

4. Have a good record of paying taxes and social security funds in accordance with the law.

5. In the three years before participating in government procurement activities, there are no major illegal records in business activities.

6. Other conditions stipulated by laws and administrative regulations.

6. Inquiry schedule

1. Inquiry release time: 2026-08-31 10:00

2. Inquiry deadline: 2026-09-03 10:00

Suppliers should encrypt and compress the quotation list and related attachments (which need to be stamped with the official seal and scanned in color) into a zip format before the quotation deadline, and only send the encrypted compressed package to the Jinfeng Laboratory procurement email jfsys-caigou@jflab.ac.cn. The decompression password and project quotation amount must not be entered in the body of the email, otherwise it will be treated as an invalid quotation. The password will be notified separately after the expiration date by the purchaser. Those who do not quote before the quotation deadline will be deemed to have given up automatically. The subject of the email must be filled in strictly in accordance with the format: [Full name of the project] + Supplier’s complete unit name + Quotation document. If the subject is filled in incorrectly, causing the purchaser to be unable to identify and classify the email, and the risk of the email being misjudged as spam by the system is borne by the supplier.

7. Quotation document requirements

1. The supplier’s quotation document should contain the following attachments:

(1) Quotation letter

(2) Quotation list

(3) Legal representative qualification certificate (if the bidder is an authorized representative, he must also provide a legal representative authorization letter)

(4) Statement

(5) Letter of commitment

(6) Business license or other organizational entity certification documents

2. The bidder should prepare a freshly stamped quotation document (PDF) and send it to the mailbox. The quotation document must be clearly visible, otherwise the quotation will be deemed invalid.

3. The quotation document should be produced in accordance with the requirements of the "Purchase Announcement". The quotation document must be signed and sealed by the legal representative of the bidder or his authorized representative at the prescribed signature place. The signature, seal and content should be complete. If there are any omissions, you will bear the risk of being deemed an invalid quotation.

4. The quotation document should be written clearly and neatly. Any interline insertions, alterations, additions or deletions must be signed or stamped with a personal seal by the legal representative of the bidder or his authorized representative. Quotation documents that are illegible, unclearly expressed, or may lead to non-unique understanding will be deemed invalid.

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